Evaluation of the quality of cryopreserved umbilical cord blood before and after cryostorage BioArchive® in Stem cell bank of Moscow

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Abstract

The analysis of the quality of cryopreserved umbilical cord blood before and after the cryostorage BioArchive® of Stem Cells Bank in the Moscow. Represented the biological characteristics of cryopreserved umbilical cord blood (cell structure, cell viability (CD45+7-ADD-), the number of CD34 + -cells), harvested for clinical use. It is shown that after the cryostorage quality of cryopreserved umbilical cord blood is stored, does not change and does not depend on the long-term of storage, there is a slight decrease in the number of nucleated cells, colony-forming activity. Revealed that the preservation of hematopoietic stem cells of umbilical cord blood after cryostorage depends on the method of their selection, the initial levels of hematocrit to store and CPDA in cord blood.

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About the authors

I. V Kobzeva

Stem Cell Bank of the Moscow Health Department, Moscow, Russia

T. A Astrelina

Stem Cell Bank of the Moscow Health Department, Moscow, Russia

M. V Yakovleva

Stem Cell Bank of the Moscow Health Department, Moscow, Russia

E. E Karpova

Stem Cell Bank of the Moscow Health Department, Moscow, Russia

Y. A Kruglova

Stem Cell Bank of the Moscow Health Department, Moscow, Russia

E. V Boyakova

Stem Cell Bank of the Moscow Health Department, Moscow, Russia

N. R Shachpazyan

Stem Cell Bank of the Moscow Health Department, Moscow, Russia

A. E Gomzyakov

Stem Cell Bank of the Moscow Health Department, Moscow, Russia

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