The cytofluorimetric characteristics of RNAse influence towards pro- and eucariotic cells

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Cytotoxic ribonucleases (RNAses) are known to be
perspective drugs for cancer therapy. The extension of model
objects range will give possibility to assess the cytotoxic
RNAses selectivity.
We estimated the effect of Bacillus intermedius
ribonuclease (binase) and bovine RNAse A to E. coli K 12 and
lung epithelia of cow embryo (LEC) cells. LEC cells apoptosis
was characterized with a double staining with annexin-FITC and
propidium iodide (PI). E. coli K12 cells vitality was estimate
via PI staning.
Binase and RNAse A were not cytotoxic to LEC and
E. coli K12 cells in investigated concentrations (100 and
300 ƒg/ml).
Low RNAses toxicity for E. coli allows to suppose the
binase and RNAse A in concentrations, capable to display
antitumor activity, will not to effect the tissues microbial
flora during in vivo tests. The lack of apoptosis inducing
activity of binase for LEC cells confirms this RNAse selectivity
for tumor cells.

About the authors

P V Zelenikhin,

Kazan (Volga region) Federal University, Kazan

Kazan (Volga region) Federal University, Kazan

K R Mamedzade,

Kazan (Volga region) Federal University, Kazan

Kazan (Volga region) Federal University, Kazan

O N Ilinskaya

Kazan (Volga region) Federal University, Kazan

Kazan (Volga region) Federal University, Kazan


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