Gene transfer using new complexes between cardiolipin-like dicationic lipids and plasmid DNA to tumor cells
- Authors: Zhdanov RI1,2,3, Moskovtsev AA2,4,3, Blokhin DY.4, Doynikova AN5, Shakirova RI5, Sebyakin Y.L6
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Affiliations:
- Kazan (Volga region) Federal University, Kazan
- Institute General Pathology and Pathophysiology of RAMS, Moscow
- Blokhin Russian Cancer Research Center of RAMS, Moscow
- Кazan (Volga region) Federal University, Kazan
- Moscow State University of Fine Chemical Technology, Moscow
- Issue: Vol 7, No 3 (2012)
- Pages: 57-61
- Section: Articles
- URL: https://genescells.ru/2313-1829/article/view/121583
- DOI: https://doi.org/10.23868/gc121583
- ID: 121583
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Abstract
The lipid vesicles of bisamphiphiles cardiolipin-like dicationic
lipids (CDL) I-IV were studied for creation of lipoplexes with
plasmid DNA of different sizes to obtain stable lipoplexes for
gene transfer to gene therapy. Lipoplexes sizes (300±100 nm)
and stablity (> 2 hrs) of CDL were sufficient to be used in
gene transfer against monolayer and suspension cell cultures.
The CDL total cytotoxicity determined by MTT-test was lower
compare to lipofectin as a control. Transfection conditions
against tumor cells lines were optimized by lipoplexes of CDL
and plasmid DNA. The most efficient transfection for lipoplexes
CDL-plasmid DNA was at the lipid-DNA (L/D) ratio equal to 5
(for lipofectin, it was 2). For monolayer cell cultures, lipoplexes
CDL-I are comparable in terms of transfection efficacy with
lipofectin; in the case of suspension culture, their efficiency
was lower by one order of magnitude. It permits a usage
of lipoplexes suggested as mediators for gene transfer and
delivery to human tumor cells.
lipids (CDL) I-IV were studied for creation of lipoplexes with
plasmid DNA of different sizes to obtain stable lipoplexes for
gene transfer to gene therapy. Lipoplexes sizes (300±100 nm)
and stablity (> 2 hrs) of CDL were sufficient to be used in
gene transfer against monolayer and suspension cell cultures.
The CDL total cytotoxicity determined by MTT-test was lower
compare to lipofectin as a control. Transfection conditions
against tumor cells lines were optimized by lipoplexes of CDL
and plasmid DNA. The most efficient transfection for lipoplexes
CDL-plasmid DNA was at the lipid-DNA (L/D) ratio equal to 5
(for lipofectin, it was 2). For monolayer cell cultures, lipoplexes
CDL-I are comparable in terms of transfection efficacy with
lipofectin; in the case of suspension culture, their efficiency
was lower by one order of magnitude. It permits a usage
of lipoplexes suggested as mediators for gene transfer and
delivery to human tumor cells.
Keywords
About the authors
R I Zhdanov
Kazan (Volga region) Federal University, Kazan;Institute General Pathology and Pathophysiology of RAMS, Moscow;Kazan (Volga region) Federal University, Kazan;Institute General Pathology and Pathophysiology of RAMS, Moscow;
A A Moskovtsev
Institute General Pathology and Pathophysiology of RAMS, Moscow;Blokhin Russian Cancer Research Center of RAMS, Moscow;Institute General Pathology and Pathophysiology of RAMS, Moscow;Blokhin Russian Cancer Research Center of RAMS, Moscow;
D Yu Blokhin
Blokhin Russian Cancer Research Center of RAMS, MoscowBlokhin Russian Cancer Research Center of RAMS, Moscow
A N Doynikova
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
R I Shakirova
Кazan (Volga region) Federal University, KazanКazan (Volga region) Federal University, Kazan
Yu L Sebyakin
Moscow State University of Fine Chemical Technology, MoscowMoscow State University of Fine Chemical Technology, Moscow
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