Properties of tissue-engineering polycaprolactone matrices impregnated by VEGF and bFGF growth factors

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A contemporary approach to small vascular conduits design for bypass surgery is growing them in vivo using tissue-engineered biodegradable polymer scaffolds. The study assessed the possibility to use grafts made by two-phase electrospinning out of polycaprolactone with impregnated VEGF and bFGF. The scaffolds were tested for the alterations in physical, mechanical and biological properties after their impregnation with the growth factors. An increase in polymer graft strength was observed following their impregnation with VEGF and bFGF. ELISA showed a prolonged biomolecule release out of the scaffold within up to 3 weeks. The results of subcutaneous implantation of the scaffolds to Wistar rats demonstrated that the biological activity of VEGF and bFGF is preserved after their release into the surrounding tissues. Thus, the study showed that there is a possibility to use PCL scaffolds with VEGF and bFGF to design small vascular tissue- engineered grafts.

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About the authors

V. V Sevostyanova

Institute for Complex Problems of Cardiovascular Disease of SB RAMS, Kemerovo

Y. L Elgudin

Case Western Reserve University, Cleveland, USA

G. E Wnek

Case Western Reserve University, Cleveland, USA

T. Lubysheva

Case Western Reserve University, Cleveland, USA

S. Emancipator

Case Western Reserve University, Cleveland, USA

A. S Golovkin

Institute for Complex Problems of Cardiovascular Disease of SB RAMS, Kemerovo

L. S Barbarash

Institute for Complex Problems of Cardiovascular Disease of SB RAMS, Kemerovo


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