Characteristics of placental multipotent mesenchymal stromal stem cells

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Abstract

In this paper we have shown the presence of population of cytokeratyn-7-producing cells in culture of multipotent mesenchymal stromal cells (MMSC] from placenta with immunophenotype CD90 +/CD73 +/CD105 +/HLA -ABD ow/CD34 -/ CD45 -/CD133 -/CD14 -. It was shown by immunocytochemistry of placental MMCS a significant decrease of the population of cytokeratyn-7 positive cells during three passages in vitro. A chimerism of mother cells in cell culture of placental MMSC was established. PCR analysis has detected expression of transcription factors genes which are typical for embryonic and heart stem cells OCT-4 and NKX2-5, and at first the expression of genes sppl, col2a1 and ppar-y2 in cell cultures of placental MMSC with adipogenic, osteogenic and chondrogenic potential in vitro was described. Also we showed the necessity of individual selection of marker genes for differentiation of MMCS from various sources, depending on their initial level of expression.

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About the authors

V. Shablii

Institute of Molecular Biology and Genetics of NAS of Ukraine, Kiev, Ukraine; Institute of Cell Therapy, Kiev, Ukraine

M. Kuchma

Institute of Molecular Biology and Genetics of NAS of Ukraine, Kiev, Ukraine; Institute of Cell Therapy, Kiev, Ukraine

V. Kyryk

State Institute of Genetic and Regenerative Medicine of NAMS of Ukraine, Kiev, Ukraine

G. Onishchenko

Institute of Cell Therapy, Kiev, Ukraine; Institute of Biology of the T. Shevchenko National University of Kiev, Kiev, Ukraine

P. Areshkov

Institute of Molecular Biology and Genetics of NAS of Ukraine, Kiev, Ukraine

N. Skrypnyk

Institute of Biology of the T. Shevchenko National University of Kiev, Kiev, Ukraine

L. Lukash

Institute of Molecular Biology and Genetics of NAS of Ukraine, Kiev, Ukraine

G. Lobyntseva

Institute of Cell Therapy, Kiev, Ukraine

References

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