Effect of simultaneous expression of various usoforms of vascular endothelial growth factor VEGF and fibroblast growth factor FGF2 on proliferation of human umbilical cord blood cells HUVEC



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Abstract

Transplantation of stem cells, progenitor and differentiated cells is currently actively investigated as an approach for correcting degenerative diseases. However the mechanisms of therapeutic effect of cell therapy remains poorly understood. Nowadays a theory of paracrine stimulation of regeneration processes by transplanted cells through secretion of trophic and growth factors gains a particular popularity. Genetic modification of cells prior to transplantation allows efficient expression and targeted delivery of various therapeutic factors. The use of plasmid expression vectors are considered to be one of the safest and promising approaches for genetic modification of cells. We report generation of genetic constructs based on expression plasmid pBudCE4.1 [containing two independent expression cassettes] encoding different isoforms of human vascular endothelial growth factor (VEGFJ and basic fibroblast growth factor CFGF2). Expression of recombinant proteins was confirmed by immunoblotting and immunochistochemistry. We demonstrated that genetic modification of model cell line HEK293 by our expression plasmids resulted in paracrine stimulation of human umbilical vein endothelial cells CHUVECJ proliferation in vitro.

About the authors

I I Salafutdinov

A K Shafigullina

M E Yalvach

N V Kudryashova

M A Lagar'kova

M V Shutova

S P Kiselev

R F Masgutov

R I Zhdanov

A L Kiyasov

P P Islamov

A A Rizvanov

I I Salafutdinov

Kazan Federal University, Kazan

Kazan Federal University, Kazan

A K Shafigullina

Kazan State Medical University, Kazan

Kazan State Medical University, Kazan

M E Yalvac

Yeditepe University, Istanbul, Turkey

Yeditepe University, Istanbul, Turkey

N V Kudryashova

Kazan Federal University, Kazan

Kazan Federal University, Kazan

M A Lagarkova

Vavilov Institute of General Genetics RAS, Moscow«LKT» Ltd., Moscow

Vavilov Institute of General Genetics RAS, Moscow«LKT» Ltd., Moscow

M V Shutova

Vavilov Institute of General Genetics RAS, Moscow«LKT» Ltd., Moscow

Vavilov Institute of General Genetics RAS, Moscow«LKT» Ltd., Moscow

S L Kiselev

Vavilov Institute of General Genetics RAS, Moscow

Vavilov Institute of General Genetics RAS, Moscow

R E Masgutov

Kazan Federal University, KazanKazan State Medical University, Kazan

Kazan Federal University, KazanKazan State Medical University, Kazan

R I Zhdanov

Kazan Federal University, KazanYeditepe University, Istanbul, TurkeyInstitute of general pathology and pathophysiology RAMS, Moscow

Kazan Federal University, KazanYeditepe University, Istanbul, TurkeyInstitute of general pathology and pathophysiology RAMS, Moscow

А Р Kiyasov

Kazan State Medical University, Kazan

Kazan State Medical University, Kazan

R R Islamov

Kazan State Medical University, Kazan

Kazan State Medical University, Kazan

A A Rizvanov

Kazan Federal University, KazanKazan State Medical University, KazanYeditepe University, Istanbul, Turkey

Kazan Federal University, KazanKazan State Medical University, KazanYeditepe University, Istanbul, Turkey

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