Capacity of bone marrow granylocyte and macrophage precursors in mice of different strains for in vitro colony formation under changed thymuline level in the organism and cell cultures



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Abstract

Thymiline/thymic serum factor is a highly active thymic hormone, which increases the ability of progenitors and mature T-lymphocytes to produce factors that affect hematopoiesis. The present experiments were undertaken to investigate the linear differences in the number and type of bone marrow colonies formed by the granulocyte and macrophage precursors under changed thymuline level in the organism and cell cultures. Young CBA/Ca (genotype H-2k) and FVB/N (genotype H-2q) male mice were the object of our research. In the intact, sham-operated and thymectomized mice, blood thymuline level, number of СD4+-, СD8+-Т-lymphocytes and progenitor cells for granulocyte-macrophage colonies were measured in the bone marrow. Also, changes of percent ratios of granulocytic, mixed and macrophagal blood-forming colonies under influence of thymuline in vitro and phagocytic activities of macrophages in the spleen were registered. The number of progenitor cells for granulocyte-macrophage colonies was found to be higher (p<0.05) in sham-operated versus intact mice of both strains. Thymectomy led to further increase of their number in СВА/Са mice and decrease in FVB/N mice. After thymectomy, СD4+-Т cells number decreased in СВА/Са mice whereas СD8+ Т cells in FVB/N mice. Blood thymuline level decreased after sham operation (p<0.05) only in СВА/Са mice. The activity of macrophages decreased essentially after thymectomy in СВА/Са mice and after sham operation in FVB/N mice. In sham-operated СВА/ Са mice, the ratio of blood-forming colonies was changed: the number of macrophagal colonies increased 5.8-fold compared to intact mice. This effect leveled in thymectomized mice. We observed a dose-dependent decrease in the number of macrophagal colonies under thymuline effect in vitro. In FVB/N mice the number of macrophagal colonies decreased significantly after thymectomy. However it increased under thymuline influence in vitro. Thus, the linear differences in capacity of bone marrow blood-forming cells to form colonies and be differentiated under conditions of changed functioning of the thymus can be largely linked with the specifics of its intra- and inter-systemic relationships.

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About the authors

I. F Labunets

State Institute of Genetic and Regenerative Medicine, NAMS of Ukraine

Email: irina_labunets@ukr.net

A. E Rodnichenko

State Institute of Genetic and Regenerative Medicine, NAMS of Ukraine

R. G Vasyliev

State Institute of Genetic and Regenerative Medicine, NAMS of Ukraine

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