Plakophilin-2 gene knockout using CRISPR/Cas9 system



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Abstract

CRISPR/Cas9 technology opens up broad prospects for genome manipulations. Generation of gene knockout cell lines is one of the applications of this system. This approach allows to reveal previously unknown gene functions and to reproduce the effect of pathogenic mutations. The purpose of this study was to generate plakofilin-2 gene knockout system and assess its suitability for the study of the functional effect of this knockout.

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About the authors

A. A Khudiakov

Federal V.A. Almazov North West Medical Research Centre

Saint-Petersburg, Russia

DA. A Kostina

Federal V.A. Almazov North West Medical Research Centre

Saint-Petersburg, Russia

A. A Kostareva

Federal V.A. Almazov North West Medical Research Centre

Saint-Petersburg, Russia

A. B Malashicheva

Federal V.A. Almazov North West Medical Research Centre; Staint-Petersburg State University

Email: amalashicheva@gmail.com
Saint-Petersburg, Russia

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