Development of tissue-engineered chitosan-polycaprolactone blends for vascular surgery

  • Authors: Zakharova I.S1,2,3, Smirnova A.M4,2,5, Zhiven' M.K1,2,3, Saaya S.B5, Shevchenko A.I2,6,1,7, Zakian S.M2,6,1,7, Ivanova L.N4,8
  • Affiliations:
    1. E.N. Meshalkin Siberian Federal Biomedical Research Center
    2. Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS
    3. Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the RAS.
    4. Novosibirsk State University
    5. E.N. Meshalkin Siberian Federal Biomedical Research Center.
    6. Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the RAS
    7. Novosibirsk State University.
    8. Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS.
  • Issue: Vol 11, No 4 (2016)
  • Pages: 50-56
  • Section: Articles
  • URL: https://genescells.ru/2313-1829/article/view/120577
  • DOI: https://doi.org/10.23868/gc120577

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Abstract

Tissue engineering provides the opportunity to minimize some possible negative results of the synthetic vascular grafts in long-term follow-up. The choice of the optimal scaffold and cell source for seeding are key conditions to bring properties of vessel substitute to physiological. In some works it is shown that a chitosan-polycaprolactone blend is a suitable biodegradable material for tissue engineering. In this paper we suggest an efficient method to generate of tissue-engineered chitosan-polycaprolactone blends, cellularized by endothelial cells of human cardiac explants. The cells cultured on the blended membranes retain their functional properties: viability and proliferative properties; maintain specific endothelial antigens and synthesis of extracellular matrix. These results suggested that tissue-engineered chitosan-polycaprolactone blends seeded by endothelial cells of human cardiac explants may be potential to development of substitutes for small diameter blood vessels.

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About the authors

I. S Zakharova

E.N. Meshalkin Siberian Federal Biomedical Research Center;Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS; Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the RAS.

Novosibirsk, Russia

A. M Smirnova

Novosibirsk State University;Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS;E.N. Meshalkin Siberian Federal Biomedical Research Center.

Novosibirsk, Russia

M. K Zhiven'

E.N. Meshalkin Siberian Federal Biomedical Research Center;Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS;Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the RAS.

Novosibirsk, Russia

Sh. B Saaya

E.N. Meshalkin Siberian Federal Biomedical Research Center.

Новосибирск, Россия.

A. I Shevchenko

Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS;Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the RAS;E.N. Meshalkin Siberian Federal Biomedical Research Center; Novosibirsk State University.

Novosibirsk, Russia

S. M Zakian

Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS;Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the RAS;E.N. Meshalkin Siberian Federal Biomedical Research Center; Novosibirsk State University.

Novosibirsk, Russia

L. N Ivanova

Novosibirsk State University; Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the RAS.

Novosibirsk, Russia

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