Hepato-specific small-dispersioned matrix as the important component of implanted cell-engineering designs for an auxiliary liver

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Abstract

Biocompatible and biodegraded matrixes have already framed by the modern biomedical technologies however working out and application of tissue-specific matrixes remains an actual problem of the modern tissue engineering Aim of this work is to show that the technique, which is proposed by the authors for producing of small-dispersioned matrices of decellularization liver (DCL), is good for the making of cell-engineering designs of auxiliary liver The paper presents the technology of producing the small-dispersioned matrix of DCL and the results of using the light, phase- contrast and electron microscopy to characterize the biological properties of the made matrix The hepatospecific properties of the matrix have been studied by using a quantitative evaluation of the MTT-test results on the 5th day of separate cultivation on this matrix 4 types of cells (HepG2, renal epithelial cells, bone marrow MSCs and liver cells from allogeneic donors). On photographs of microscopic examination of the matrix particles of DCL it was seen their porous structure, on the surface of which the preserved conglomerates of native extracellular matrix molecules were presented At comparative study of adhesive properties of the Cytodex-3 matrix particles and the small-dispersioned matrix of DCL it was found out that both matrices had the ability to adhere different cell types, but the matrix of DCL had the ability to preserve of hepato-specific activity significantly expressed. Keeping of biocompatibility and hepato-specific properties by small-dipersioned matrices of DCL, produced on the proposed technology, allows to recommend them for the making of implantable cell-engineering designs of auxiliary liver.

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About the authors

N. A Onischenko

Academican V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs

Email: allanik64@yandex.ru

M. E Krasheninnikov

I.M. Sechenov First Moscow State Medical University

M. Y Shagidulin

Academican V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs; I.M. Sechenov First Moscow State Medical University

M. M Bodrova

M.V. Lomonosov Moscow State University

V. I Sevastjanov

Academican V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs

S. V Gautier

Academican V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs; I.M. Sechenov First Moscow State Medical University

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