Decellularized rat heart matrix as a basis for creation of tissue engineered heart

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Abstract

Development of bioengineered scaffolds of internal organs is one of the priority areas of tissue engineering. Decellularization allows to obtain biological (natural) scaffolds while preserving extracellular matrix and three-dimensional structure of organs. The primary goals of the present research were to investigate pathological characteristics of the decellularized rat heart scaffold and evaluate adhesion and viability of multipotent mesenchymal stromal cells (MMSC) during recellularization. Rat hearts were decellularized using a modified detergent-enzymatic method including sodium deoxycholate and DNAse. The results of morphological studies have confirmed the safety of extracellular matrix proteins and patency of the coronary vessels. Mechanical testing of decellularized and native samples of rat heart showed an increase of mechanical properties of the matrix during decellularization. During the conducted experiments on recellularization of obtained scaffold has been shown that the extracellular matrix was not toxic for MMSC which were viable and maintained their metabolic activity during prolonged cultivation on the scaffold.

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About the authors

A. S Sotnichenko

Kuban State Medical University

Krasnodar

E. A Gubareva

Kuban State Medical University

Krasnodar

I. V Gilevich

Kuban State Medical University

Krasnodar

E. V Kuevda

Kuban State Medical University

Krasnodar

S. V Krasheninnikov

NRC «Kurchatov Institute»

Moscow

T. E Grigoriev

NRC «Kurchatov Institute»

Moscow

S. N Chvalun

NRC «Kurchatov Institute»

Moscow

P. Macchiarini

Kuban State Medical University; Karolinska Institutet, Stockholm

Sweden

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