Effect of tescalcin overexpression on osteogenic differentiation of human mesenchymal stem cells


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Abstract

Tescalcin plays an important role in the proliferation and differentiation of certain cell types. It is involved in the signaling pathways of mitogen-activated protein kinase and is reported to directly interact with subunit 4 of COP9 signalosome and Na/H exchanger NHE1. Since tescalcin is one of the factors that allow one type of progenitor cells differentiate into a variety of specialized cell types, it can be a potential molecular tool for modulation of phenotype of target cells. The ability of adipose-derived mesenchymal stem cells (ADSCs) to differentiate into osteogenic direction makes them a promising adult stem cell type for regenerative medicine and tissue engineering. Here we show that ADSCs with tescalcin overexpression had a significantly higher level of matrix mineralization compared with control ADSCs. This finding indicates that ectopic tescalcin overexpression might affect osteogenic differentiation of ADSCs.

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About the authors

V. V Solovyeva

Kazan (Volga region) Federal University

K. G Kolobynina

Kazan (Volga region) Federal University

M. O Gomzikova

Kazan (Volga region) Federal University

L. G Tazetdinova

Kazan (Volga region) Federal University

M. N Zhuravleva

Kazan (Volga region) Federal University

V. Z Slepak

University of Miami Miller School of Medicine

A. A Rizvanov

Kazan (Volga region) Federal University

Email: Albert.Rizvanov@kpfu.ru

References

  1. Perera E.M., Martin H., Seeherunvong T. et al. Tescalcin, a novel gene encoding a putative EF-hand Ca(2+)-binding protein, Col9a3, and renin are expressed in the mouse testis during the early stages of gonadal differentiation. Endocrinology 2001; 142(1): 455-63.
  2. Perera E.M., Bao Y., Kos L. et al. Structural and functional characterization of the mouse tescalcin promoter. Gene 2010; 464(1-2): 50-62.
  3. Levay K., Slepak V.Z. Tescalcin is an essential factor in megakaryocytic differentiation associated with Ets family gene expression. J. Clin. Invest. 2007; 117(9): 2672-83.
  4. Levay K., Slepak V.Z. Regulation of Cop9 signalosome activity by the EF-hand Ca2+-binding protein tescalcin. J. Cell. Sci. 2014; 127(Pt 11): 2448-59
  5. Mailänder J., Müller-Esterl W., Dedio J. Human homolog of mouse tescalcin associates with Na(+)/H(+) exchanger type-1. FEBS Lett. 2001; 507(3): 331-5.
  6. Li X., Liu Y., Kay C.M. et al. The Na+/H+ exchanger cytoplasmic tail: structure, function, and interactions with tescalcin. Biochem. 2003; 42(24): 7448-56
  7. Kato J.Y., Yoneda-Kato N. Mammalian COP9 signalosome. Genes Cells. 2009; 14(11): 1209-25.
  8. Malo M.E., Fliegel L. Physiological role and regulation of the Na+/H+ exchanger. Can. J. Physiol. Pharmacol. 2006; 84(11): 1081-195.
  9. Xu C., Zheng Z., Fang L. et al. Phosphatidylserine enhances osteogenic differentiation in human mesenchymal stem cells via ERK signal pathways. Mater. Sci. Eng. C. Mater. Biol. Appl. 2013; 33(3): 1783-8.
  10. Масгутов Р.Ф., Богов А.А., Ризванов А.А. и др. Стволовые клетки из жировой ткани - биологические свойства и перспективы клинического применения. Практическая медицина 2011; 7(55): 18-20.
  11. Масгутов Р.Ф., Салихов Р.З., Ризванов А.А. и др. Применение клеток стромальной васкулярной фракции жировой ткани при лечении полнослойного дефекта гиалинового хряща медиального мыщелка бедренной кости коленного сустава: клинический случай Гены и клетки 2014; 9(3): 303-6.
  12. Масгутов Р.Ф., Салихов Р.З., Плаксейчук Ю.А. и др. Применение клеток стромальной васкулярной фракции жировой ткани при ложном суставе бедренной кости: клинический случай. Клеточная трансплантология и тканевая инженерия 2013; 8(3): 116-8.
  13. Соловьева В.В., Исаев А.А., Генкин Д.Д. и др. Влияние рекомбинантного гистона Н1. 3 на эффективность лентивирусной трансдукции клеток человека in vitro. Клеточная трансплантология и тканевая инжененрия 2012; 7(3): 151-4.
  14. Solovyeva V.V., Salafutdinov I.I., Tazetdinova L.G. et al. Genetic Modification of Adipose Derived Stem Cells with Recombinant Plasmid DNA pBud-VEGF-FGF2 Results in Increased of IL-8 and MCP-1 Secretion. J. pure and applied microbiol. 2014; 8(Spl. Edn. 2): 523-8
  15. Solovyeva V.V., Salafutdinov I.I., Martynova E.V. et al. Human adipose derived stem cells do not alter cytokine secretion in response to the genetic modification with pEGFP-N2 plasmid DNA. World Applied Sciences J. 2013; 26(7): 968-72.
  16. Levay K., Slepak V.Z. Up- or downregulation of tescalcin in HL-60 cells is associated with their differentiation to either granulocytic or macrophage-like lineage. Exp. Cell. Res. 2010; 316(7): 1254-62.
  17. Jaiswal R.K., Jaiswa N., Bruder S.P. et al. Adult human mesenchymal stem cell differentiation to the osteogenic or adipogenic lineage is regulated by mitogen-activated protein kinase. J. Biol. Chem. 2000; 275(13): 9645-52.

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