Effect of tescalcin overexpression on osteogenic differentiation of human mesenchymal stem cells


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Abstract

Tescalcin plays an important role in the proliferation and differentiation of certain cell types. It is involved in the signaling pathways of mitogen-activated protein kinase and is reported to directly interact with subunit 4 of COP9 signalosome and Na/H exchanger NHE1. Since tescalcin is one of the factors that allow one type of progenitor cells differentiate into a variety of specialized cell types, it can be a potential molecular tool for modulation of phenotype of target cells. The ability of adipose-derived mesenchymal stem cells (ADSCs) to differentiate into osteogenic direction makes them a promising adult stem cell type for regenerative medicine and tissue engineering. Here we show that ADSCs with tescalcin overexpression had a significantly higher level of matrix mineralization compared with control ADSCs. This finding indicates that ectopic tescalcin overexpression might affect osteogenic differentiation of ADSCs.

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About the authors

V. V Solovyeva

Kazan (Volga region) Federal University

K. G Kolobynina

Kazan (Volga region) Federal University

M. O Gomzikova

Kazan (Volga region) Federal University

L. G Tazetdinova

Kazan (Volga region) Federal University

M. N Zhuravleva

Kazan (Volga region) Federal University

V. Z Slepak

University of Miami Miller School of Medicine

A. A Rizvanov

Kazan (Volga region) Federal University

Email: Albert.Rizvanov@kpfu.ru

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