Expression of myogenesis genes by gingiva derived cells



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Abstract

The ability of gingiva derived mesenchymal stromal cells (MSCs) to myogenic differentiation and the possibility of their expansion in vitro revealed in our previous work, open up prospects for their use in regenerative medicine for the correction of muscle pathology. Data on changes in the expression of myogenic regulatory factors in gingiva derived MSCs can serve as confirmation of the possibility of differentiation of these cells into the myogenic direction The study was conducted on cultures of gingiva derived MSCs and skin fibroblasts. When comparing gene expression profiles of gingiva derived MSCs and skin fibroblasts significant changes have been registered for 153 genes. Of these, we selected 19 significant differentially expressed genes, for which main signal pathways were analyzed. Evidences of significant activation / inactivation of signaling pathways and key genes that have been identified for the population of human satellite cells were obtained during analysis of the genome transcriptome profiles of gingiva derived MSCs This applies to such signaling pathways as the p38 MAPK, NOTCH and other groups responsible for the proliferation and differentiation in myogenic direction, the processes of self-renewal or self-maintenance in the population of satellite cells. At the same time full match in the patterns of gene expression between the stable population of gingiva derived MSCs and human satellite cells undergoing myogenic differentiation as well as cells derived from atypical sources and differentiated in the myogenic direction was not observed The data of our experiment suggests that the gingiva derived MSCs are distinct from satellite cells and could be considered as population with plateaued development, possessing the ability to differentiate into myogenic direction due to non-canonical expression pattern of myogenic regulatory factors

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About the authors

D. P Samchuk

Central Clinical Hospital with Outpatient Health Center

E. N Lukyanova

Human Stem Cells Institute

I. I Eremin

Central Clinical Hospital with Outpatient Health Center

V. L Zorin

Central Clinical Hospital with Outpatient Health Center; Human Stem Cells Institute

A. I Zorina

Human Stem Cells Institute

O. S Grinakovskaya

Central Clinical Hospital with Outpatient Health Center

I. N Korsakov

Central Clinical Hospital with Outpatient Health Center

R. V Deev

Kazan (Volga region) Federal University

I. R Gilmutdinova

Central Clinical Hospital with Outpatient Health Center

N. L Lazareva

Central Clinical Hospital with Outpatient Health Center

P. S Eremin

Central Clinical Hospital with Outpatient Health Center

A. P Petrikina

Central Clinical Hospital with Outpatient Health Center

A. E Gomzyakov

Central Clinical Hospital with Outpatient Health Center

D. A Timashkov

Central Clinical Hospital with Outpatient Health Center

N. K Vit’ko

Central Clinical Hospital with Outpatient Health Center

K. V Kotenko

Central Medical Authority of the Business Administration for the President of the Russian Federation

P. B Kopnin

N.N. Blokhin Cancer Research Center

A. A Pulin

Central Clinical Hospital with Outpatient Health Center

Email: andreypulin@gmail.com

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