Influence of allogeneic hematopoietic stem/ progenitor cells treated with IL-3 on structural-functional heart changes under experimental postinfarction heart failure in long-term period of observation


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Abstract

The aim of this research was to study the influence of allogeneic hematopoietic stem/ progenitor cells infused intravenously to rats in acute period of izoproterenol-induced myocardial infarction on structural and functional changes of the heart in long-term period of observation (over 1 year) The postinfarction heart failure was created by intra-abdominal injection of isoproterenol solution Allogenic CD117+ hematopoietic stem/ progenitor cells were extracted from bone marrow by immunomagnetic separation and were induced for CXCR4 expression and TGFb production by cultivation for three days in presence of interleukine-3. Obtained cells were injected intravenously to rats on 9 day after isoproterenol administration. One year later structure and function of the myocardium were investigated by epicardial echocardiography in M-modal mode with the apparatus «VIVID-3» supplied by 3. 5 MHz transduser The diastolic function was examined by Doppler echocardiography method in the impulse mode One year later, in the group of infarcted animals with hematopoietic stem/ progenitor stem cell injection the clinically and statistically significant reduction of the left ventricle weight and relative thickness of the left ventricle wall were revealed; furthermore - the reduction of the left ventricle back wall thickness, interventricular septum thickness and aorta diameter were observed in comparison with the group without treatment Doppler echocardiographic test has demonstrated an improvement of cardiac diastolic function in the group of infarcted animals with hematopoietic stem/ progenitor cell injection: statistically significant reduction of proportion of the animals with hypertrophic and restrictive mode of transmitral blood flow Obtained data have shown that single intravenous injection of hematopoietic stem/ progenitor cells treated with IL-3 ex vivo reduces a risk of serious cardiovascular complications after myocardial infarction at long-term period of observation in rats Proposed approach may be useful in improving of cell therapy effectiveness for heart failure treatment

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About the authors

E. A Severova

Research Institute of Cardiology and Internal Medicine

Email: severovalena@yandex ru

S. F Berkinbayev

Research Institute of Cardiology and Internal Medicine

M. N Nugmanova

Research Institute of Cardiology and Internal Medicine

N. M Pominova

Research Institute of Cardiology and Internal Medicine

Yu. V Perfilyeva

M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry

T. A Supniyazova

M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry

Yu. D Denisov

Research Institute of Cardiology and Internal Medicine

N. N Belyaev

M.A. Aitkhozhin Institute of Molecular Biology and Biochemistry

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