Expression of pluripotency transcription factors in human third molar tooth germ derived multipotent mesenchymal stromal cells transfected by plasmid pBud-Sox2-Oct4


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Abstract

In this study, the double expression cassette plasmid, based on pBudCE4 1 vector encoding transcription factors S0X2 and 0CT4 was constructed using standard gene engineering techniques. Expression of recombinant genes was confirmed by immunoblotting. It is shown that genetic modification of multipotent mesenchymal stromal cells (MMSC), isolated from human third molar tooth germs, with resulting recombinant plasmid increases the level of expression both, transcription factors S0X2 and OCT4 in the treated cells, and also transcription factor NAN0G. Analysis of histological sections of subcutaneous Matrigel implants, containing fluorescently labeled MMSC, showed that genetic modification had no effect on cell viability

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About the authors

V. V Solovyeva

Kazan (Volga Region) Federal University

N. L Blatt

Kazan (Volga Region) Federal University

D. S Guseva

Kazan State Medical University; Hannover Medical School

M. E Yalvac

Yeditepe University; Children’s Hospital Columbus

F. Sahin

Yeditepe University

R. R Islamov

Kazan State Medical University

A. A Rizvanov

Kazan (Volga Region) Federal University

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