Effects of Dalarginum on proliferation of multipotent mesenchymal stromal cells, dermal fibroblasts, and human osteosarcoma cells in vitro


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Abstract

The aim of this work was to assess in vitro the effect of various doses of Dalarginum and its blocker Naloxone on the proliferation of multipotent mesenchymal stromal cells (MMSC), dermal fibroblasts (FD) and human osteosarcoma cell (line HOS). We used MMSC and FD derived from three donors. The cells were cultured for 3 days with solutions of the test substances at following concentrations: Dalarginum 10 jg/l, 100 jg/l, 1000jg/l; combination of naloxone 0.5 mg/l and Dalarginum, 100 jg/l; combination of Naloxone 3 mg/l and Dalarginum, 100jg/l. Saline was added in the culture medium in the control group. The cells were counted by the end of day 3 of culturing. Dalarginum at 100 jg/l significantly increased the number of MMSC by 19-34 % and decreased the count of cells (line HOS) by 22-34 % compared with the control values. There were no significant differences in cell numbers between the groups with addition of dalarginum at 10jg/l and 1000jg/l as well as significant changes in the number of FD under the influence of the test substances. Dalarginum combined with Naloxone, opioid receptor antagonist, had no impact on the number of cells, which confirmed its receptor-mediated action. The optimum effect of Dalarginum on opioid receptors was observed at100jg/l.

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About the authors

A. V Vasilyev

Research Institute of Human Morphology of RAMS; Research Centre of Medical Genetics of RAMS

Moscow, Russia

T. B Bukharova

Research Centre of Medical Genetics of RAMS

Moscow, Russia

A. V Volkov

Research Institute of Human Morphology of RAMS

Moscow, Russia

E. B Vikhrova

Research Centre of Medical Genetics of RAMS

Moscow, Russia

G. B Bolshakova

Research Institute of Human Morphology of RAMS

Moscow, Russia

D. V Goldstein

Research Centre of Medical Genetics of RAMS

Moscow, Russia

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