Activity index of lymphocyte apoptosis in children with neonatal sepsis


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Abstract

We studied the lymphocyte apoptosis in 15 infants with late neonatal sepsis, whereas 8 of them (53.3%) were considered as a preterm infants. Etiology of sepsis was identified in 4 cases (26.7%): in 1 case of each, the cause of the disease was Klebsiella pneumoniae, Pseudomonas koreenses, Candida and St. agalactae. Control group consisted of 5 healthy newborns. Apoptosis analysis was conducted by measuring the amount of hypodiploid cells by using a propidium iodide (Sigma Aldrich) DNA staining procedure and flow cytometry (FACsCanto II, Becton Dickinson). We observed an elevated numbers of apoptotic cells in all cases of neonatal sepsis. The most significant difference was observed when the lymphocytes were cultured for 3 and 5 days. For example, on day 3 of culture the numbers of apoptotic cells in patients with neonatal sepsis vs control group were 19.6% and 5.13%, respectively. Absolute lymphopenia was noted in 26.7% of cases with neonatal sepsis. No difference in the lymphocyte apoptosis between term and preterm infants was observed. An increased amount of lymphocyte apoptosis was not associated with C-reactive protein level during the whole time-points of the experiment: at 1, 3 and 5 days. Acute phase of neonatal sepsis is associated with an increased apoptosis of peripheral blood lymphocytes.

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About the authors

Kh. S Khaertynov

Kazan State Medical University

S. V Boichuk

Kazan State Medical University

V. A Anokhin

Kazan State Medical University

B. R Ramazanov

Kazan State Medical University

P. D Dunaev

Kazan State Medical University

S. F Khaiboullina

Kazan (Volga Region) Federal University

A. A Rizvanov

Kazan (Volga Region) Federal University

A. A Andreeva

Children's Republican Clinical Hospital

M. A Satrutdinov

Children's Republican Clinical Hospital

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