Hepatic stellate cells stimulate liver regeneration after partial hepatectomy under inhibition of hepatocyte proliferation


Cite item

Full Text

Abstract

Most of the fundamental studies in the field of developing new methods for treating liver diseases in regenerative medicine are performed with haematopoietic and mesenchymal stem cells. At the same time more and more importance is gaining need for finding new approaches enabling stimulation of regional stem cell compartment and using regional stem cells which are possibly represented by hepatic stellate cells. But studies aimed at their transplantation are rare. Previously, we have established the possibility of these cells to differentiate into hepatocytes after transplantation to rats with partial hepatectomy. In our present research we studied liver regeneration after transplantation of hepatic stellate cells on partial hepatectomy with 2-acetylaminofluorene damage model in rats. 2-acetylaminofluorene blocks hepatocyte proliferation. Results of study confirmed that hepatic stellate cells have the ability to differentiate into hepatocyte and stimulate it's regeneration without the threat of fibrosis after transplantation in rats with partial hepatectomy and administration of 2-acetylaminofluorene.

Full Text

Restricted Access

About the authors

A. A Titova

Kazan (Volga region) Federal University

G. R Burganova

Kazan (Volga region) Federal University

E. I Sharipova

Kazan (Volga region) Federal University; Kazan State Medical University

G. O Pevnev

Kazan (Volga region) Federal University; Kazan State Medical University

M. O Mavlikeev

Kazan (Volga region) Federal University

I. M Gazizov

Kazan (Volga region) Federal University; Kazan State Medical University

A. R Galyavieva

Kazan (Volga region) Federal University; Kazan State Medical University

A. K Shafigullina

Kazan (Volga region) Federal University

M. S Kaligin

Kazan (Volga region) Federal University

MA. A Titova

Kazan (Volga region) Federal University

AA. A Gumerova

Kazan (Volga region) Federal University

A. P Kiassov

Kazan (Volga region) Federal University

References

  1. Higgins G.M., Anderson R.M. Experimental pathology of the liver: I. Restoration of the liver of the white rat following partial surgical removal. Arch. Pathol. 1931; 12: 186-202.
  2. Bucher N.R., Farmer S. Liver regeneration after partial hepatectomy: genes and metabolism. In: Strain A.J., Diehl A., editors. Liver growth and repair. London: Chapman and Hall, 1998; 3-27.
  3. Газизов И.М., Калигин М.С., Андреева Д.И. и др. Изменения микроструктуры печени после частичной гепатэктомии у крыс. Клеточная трансплантология и тканевая инженерия 2013; VIII (3); 101-5.
  4. Taub R. Liver regeneration: from myth to mechanism. Nat. Rev. Mol. Cell Biol. 2004; 5: 836-47.
  5. Martinez-Hernandez A., Amenta P.S. The hepatic extracellular matrix. II. Ontogenesis, regeneration and cirrhosis. Virchows Arch. Pathol. Anat. Histopathol. 1993; 423: 77-84.
  6. Solt D., Medline A., Farber E. Rapid emergence of carcinogen-induced hyperplastic lesions in a new model for the sequential analysis of liver carcinogenesis. Am. J. Pathol.; 1977; 88: 595-609.
  7. Sell S. Is there a liver stem cell? Cancer Res. 1990; 50: 3811-5.
  8. Гумерова А.А., Киясов А.П. Участие клеток Ито в гистогенезе и регенерации печени. Клеточная трансплантология и тканевая инженерия 2007; 2(4): 39-46.
  9. Урываева И.В., Цитрин Е.Б., Городецкий С.И. и др. Фенотипические признаки стволовых клеток - экспрессия мембранного транспортера Bcrp1/Abcg2 и экспорт красителя Hoechst 33342 - у гепатоцитов при регенерации печени. Доклады академии наук 2004; 398(3): 422-5.
  10. Шафигуллина А.К., Трондин А.А., Бурганова Г.Р. и др. Сравнение различных методов выделения, мечения и трансплантации звёздчатых клеток печени крыс. Клеточная трансплантология и тканевая инженерия 2013; 3: 147-51.
  11. Kordes C., Sawitza I., Muller-Marbach A. et al. CD133+ hepatic stellate cells are progenitor cells. Biochem Biophys Res Commun. 2007; 52(2): 410-7.
  12. Cui L., Shi Y., Zhou X. et al. A set of microRNAs mediate direct conversion of human umbilical cord lining-derived mesenchymal stem cells into hepatocytes. Cell Death Dis. 2013; 4: e918.
  13. Kaibori M., Adachi Y., Shimo T. et al. Stimulation of liver regeneration after hepatectomy in mice by injection of bone marrow mesenchymal stem cells via the portal vein. Transplant. Proc. 2012; 44(4): 1107-9.
  14. Khuu D.N., Nyabi O., Maerckx C. et al. Adult human liver mesenchymal stem/progenitor cells participate in mouse liver regeneration after hepatectomy. Cell Transplant. 2013; 22(8): 1369-80.
  15. Seki T., Yokoyama Y., Nagasaki H. et al. Adipose tissue-derived mesenchymal stem cell transplantation promotes hepatic regeneration after hepatic ischemia-reperfusion and subsequent hepatectomy in rats. J. Surg. Res. 2012; 178(1): 63-70.
  16. Devine S.M., Cobbs C., Jennings M. et al. Mesenchymal stem cells distribute to a wide range of tissues following systemic infusion into nonhuman primates. Blood 2003; 101(8): 2999-3001.
  17. Campard D., Lysy P.A., Najimi M. et al. Native umbilical cord matrix stem cells express hepatic markers and differentiate into hepatocyte-like cells. Gastroenterol. 2008; 134(3): 833-48.
  18. Popp F.C., Slowik P., Eggenhofer E. et al. No contribution of multipotent mesenchymal stromal cells to liver regeneration in a rat model of prolonged hepatic injury. Stem Cells 2007; 25(3): 639-45.
  19. Petersen B.E., Bowen W.C., Patrene K.D. et al. Bone marrow as a potential source of hepatic oval cells. Science 1999; 284(5417): 1168-70.
  20. Andreeva D., Gazizov I., Yilmaz T. et al. Differentiation of transfected human umbilical cord blood stem cells in the regenerating liver of rats. Abstracts of 45th annual meeting of the European association for the study of the liver. J. Hepatol. 2010; 52: 353-4.
  21. Шафигуллина А.К., Гумерова А.А., Трондин А.А. и др. Трансплантированные звёздчатые клетки печени участвуют в регенерации органа после частичной гепатэктомии без риска развития фиброза печени. Клеточная трансплантология и тканевая инженерия 2012; 3: 169-72.
  22. Газизов И.М., Калигин М.С., Андреева Д.И. и др. Изменения микроструктуры печени после частичной гепатэктомии у крыс. Клеточная трансплантология и тканевая инженерия 2013; 3: 101-5.

Copyright (c) 2014 PJSC Human Stem Cells Institute


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС 77 - 57156 от 11.03.2014.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies