Pathohistological assessment of skeletal muscle after direct gene therapy with vegf165 of patients with peripheral arterial diseases



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Abstract

The aim was to elucidate impact of gene therapy with plasmid encoding vegf165 on the muscle tissue pathohistology of patients with peripheral arterial diseases. Twice repeated intramuscular injections of plasmid («Neovaskulgen», RN LP-000671 from 28.09.2011) were performed to 6 patients (ischemia grade IIb-III by Pokrovsky-Fontaine) according to specification. Standart tredmill test, ankle-brachial index estimation were performed. Histological study of injured muscle biopsies taken before and 3 months after injection was performed. In intact muscles therapy with vegf165 leads to increase of mean cross-sectional muscle fiber area without significant angiogenic effect. In muscles with decreased capillary density this therapy leads to blood supply improvement promoting regeneration of muscles by myosatellites proliferation and increase of mean cross-sectional muscle fiber area and connective tissue degradation. Treadmill test showed painless walking distance increased by 31,74% on average (from 94,96±49,79 m to 139,11±60,78 m, p<0,05) in all patients. There was correlation of pathohistological analysis with clinical data.

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About the authors

M. O Mavlikeev

Kazan (Volga region) Federal University

M. V Plotnikov

Republic Clinical Hospital of Republic of Tatarstan; Kazan State Medical Academy

A. V Maksimov

Republic Clinical Hospital of Republic of Tatarstan; Kazan State Medical Academy

G. R Gafiyatullina

Kazan (Volga region) Federal University

A. I Murtazin

Kazan State Medical University

U. E Teregulov

Republic Clinical Hospital of Republic of Tatarstan

I. I Shamsutdinova

Republic Clinical Hospital of Republic of Tatarstan

A. A Gumerova

Kazan (Volga region) Federal University

A. A Rizvanov

Kazan (Volga region) Federal University

A. P Kiassov

Kazan (Volga region) Federal University

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