Transplantation of mononuclear cells of human umbilical cord blood improves spatial memory in APP/PS1 transgenic mice with alzheimer's disease model


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Abstract

Alzheimer's disease is progressive incurable neurodegenerative disease, which is manifested mainly by dementia. One of the most promising directions in development of Alzheimer's disease treatment is use of gene-cell technologies. The aim of current study was to perform transplantation of wild-type or EGFP expressing umbilical cord blood mononuclear cells (МКПК) to APP/PS1 transgenic mice with Alzheimer's disease model with further evaluation of transplantation impact with behavioral (T-maze, plus maze, open field) and immunohistochemical methods. It was found that МКПК transplantation significantly ameliorates behavioral performance of APP/PS1 mice: improves spatial memory, decreases anxiety and non-specific excitability, increases the efficacy of exploratory behavior. Grafted cells were found in cortex and hippocampus of mice even 3 months after МКПК transplantation, herewith EGFP expression in grafted cells was found at early stages after transplantation. Thus, use of МКПК-based gene-cell constructs represents a promising direction in development of Alzheimer's disease therapy.

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About the authors

E. O Petukhova

Kazan State Medical University

Y. O Mukhamedshina

Kazan State Medical University; Kazan (Volga region) Federal University

A. A Rizvanov

Kazan (Volga region) Federal University

A. R Mukhitov

Kazan Institute of biochemistry and biophysics of RAS

A. L Zefirov

Kazan State Medical University

R. R Islamov

Kazan State Medical University

M. A Mukhamedyarov

Kazan State Medical University

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