Proliferation of cells of the pancreas in experimental diabetes

Cite item


The proliferative process intensification in the disrupted organs is very important in reparative regeneration. But the possibility of pancreas cells proliferation during damage is not been studied. The aim of our work was to examine the proliferation activity of pancreas during the experimental alloxan diabetes. The work was made on 33 rats. Blood glucose levels were measured. Also the expression of nuclear antigen of proliferating cells PCNA was studied in pancreas cells. After the day of the experimental hyperglycemia PCNA expression in islet and acinar cells of pancreas was found, which was persisted at all stages of the experiment. After 2 days of the experiment single PCNA-positive cells with the cytoplasmic staining were found in the interstitium, near vessels and ducts. Proliferating cells, which produce insulin were found in islets during double staining. Thus, there is the proliferation in islet and acinar cells of pancreas during experimental diabetes type I after alloxan damage. Also, the results of our study confirms the ability of p-cells and progenitor pancreas cells proliferation.

Full Text

Restricted Access

About the authors

M. S Kaligin

Kazan (Volga Region) Federal University

AA. A Titova

Kazan (Volga Region) Federal University

A. S Plushkina

Kazan (Volga Region) Federal University; Kazan State Medical University

MA. A Titova

Kazan (Volga Region) Federal University

A. A Gumerova

Kazan (Volga Region) Federal University

A. P Kiassov

Kazan (Volga Region) Federal University; Kazan State Medical University


  1. Дедов И.И., Мельниченко Г.А., Фадеев В.Ф. Эндокринология. М.: ГЭОТАР-Медиа, 2007: 432.
  2. Bonner-Weir S. Beta-cell turnover: its assessment and implications. Diabets. 2001; 50: 20-4.
  3. Yamada S., Kojma I. Regenerative medicine of the pancreatic в cells. Hepatobiliary Pancreat. Surg. 2005; 12: 218-26.
  4. Орехович В.Н. Современные методы в биохимии. М.: Наука, 1977: 392.
  5. Bonner-Weir S., Toschi E., Inada A. et al. The pancreatic ductal epithelium serves as a potential pool of progenitor cells. Pediatr. Diabetes. 2004; 5: 16-22.
  6. Kim N., Yoo W., Lee J., Kim H. and all. Formation of vitamin A lipid droplets in pancreatic stellate cells requires albumin. Int. J. Gastroenterol. Hepatol. 2009; 58(10): 1382-90.
  7. Калигин М.С., Плюшкина А.С., Титова А.А. и др. С-kit и дес-мин-позитивные клетки в регенерации островков поджелудочной железы при экспериментальном диабете у крыс. Клеточная трансплантология и тканевая инженерия. 2013; VIII(3): 113-5.
  8. Титова А.А., Шарипова Э.И. Экспрессия инсулина десмин-по-зитивными звёздчатыми клетками поджелудочной железы человека. 86-я Всероссийская студенческая научная конференция памяти чл.-корр. Академии наук РТ, проф. Салихова. Казань, 2012: 241.
  9. Плюшкина А.С., Калигин М.С., Андреева Д.И., Титова А.А. и др. C-kit-позитивные клетки островков поджелудочной железы крысы как клетки-предшественницы эндокриноцитов при аллоксановом диабете. Клеточная трансплантология и тканевая инженерия 2012; VII(3): 138-41.
  10. Калигин М.С., Гумерова А.А, Титова М.А, Андреева Д.И. и др. C-kit маркёр стволовых клеток эндокриноцитов поджелудочной железы. Морфология 2011; 140 (4): 32-7.
  11. Grzanka, A., Skok Z., Janiak A., Grzanka D. The expression of proliferating cell nuclear antigen (PCNA) in leukemia cell lines HL-60 and K-562 at the light and electron microscope level. Neoplasma 2000; 47(5): 288-93.
  12. Morrow P.W., Tung H.Y., Hemmings H.C. Jr. Rapamycin causes activation of protein phosphatase-2A1 and nuclear translocation of PCNA in CD4+ T cells. Biochem. Biophys. Res. Commun. 2004; 323(2): 645-51.
  13. Fujisawa H., Zhang Z., Sun W. et al. Histopathological Changes in the Pancreas from a Spontaneous Hyperglycemic Cynomolgus Monkey. Tox. Pathol. 2012; 25(3): 215-9.

Copyright (c) 2014 PJSC Human Stem Cells Institute

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС 77 - 57156 от 11.03.2014.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies