Oral mucosais a new source for myoblast derivation



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Abstract

Leading laboratories in the world intensively research tissue-specific stem cells. The main goal of such work is development of improved technique of tissue regeneration stimulation. Optimization of conditions of differentiation induction, and search for new sources of cells are actual problems in this field of science. Human gingival mucosa is one of promising sources of cells. Gingival mucosa-derived multipotent mesenchymal stromal cells (gm MMSC) have a number of features, notably the high proliferative activity and ability to multilineage differentiation. However, their myogenic differentiation has not been proofed yet. The study was conducted on 10 gm MMSC cultures obtained from gingival mucosa biopsy samples of 10 healthy volunteers. Possibility of gm MMSC obtainment with high proliferative potential and ability of cells to efficiently differentiate not only into orthodox (adipogenic, osteogenic, chondrogenic) directions but also in myogenic direction during both early and late passages was demonstrated for the first time in our work. Our results of gm MMSC investigation and characteristics of the cell'ssource, confirm advantages of gm MMSC use in regenerative medicine, in particular for the treatment of the muscle tissue diseases of different etiology.

About the authors

V. L Zorin

Human Stem Cells Institute, Moscow, Russia; A. I. Burnazyan Federal Medical Biophysical Center FMBA of Russia, Moscow, Russia

I. I Eremin

A. I. Burnazyan Federal Medical Biophysical Center FMBA of Russia, Moscow, Russia

V. A Rybko

N. N. Blokhin Cancer Research Center, Moscow, Russia

A. I Zorina

Human Stem Cells Institute, Moscow, Russia

K. V Kotenko

A. I. Burnazyan Federal Medical Biophysical Center FMBA of Russia, Moscow, Russia

A. A Pulin

A. I. Burnazyan Federal Medical Biophysical Center FMBA of Russia, Moscow, Russia

P. B Kopnin

Human Stem Cells Institute, Moscow, Russia; N. N. Blokhin Cancer Research Center, Moscow, Russia

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