Oral mucosais a new source for myoblast derivation

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access


Leading laboratories in the world intensively research tissue-specific stem cells. The main goal of such work is development of improved technique of tissue regeneration stimulation. Optimization of conditions of differentiation induction, and search for new sources of cells are actual problems in this field of science. Human gingival mucosa is one of promising sources of cells. Gingival mucosa-derived multipotent mesenchymal stromal cells (gm MMSC) have a number of features, notably the high proliferative activity and ability to multilineage differentiation. However, their myogenic differentiation has not been proofed yet. The study was conducted on 10 gm MMSC cultures obtained from gingival mucosa biopsy samples of 10 healthy volunteers. Possibility of gm MMSC obtainment with high proliferative potential and ability of cells to efficiently differentiate not only into orthodox (adipogenic, osteogenic, chondrogenic) directions but also in myogenic direction during both early and late passages was demonstrated for the first time in our work. Our results of gm MMSC investigation and characteristics of the cell'ssource, confirm advantages of gm MMSC use in regenerative medicine, in particular for the treatment of the muscle tissue diseases of different etiology.

About the authors

V. L Zorin

Human Stem Cells Institute, Moscow, Russia; A. I. Burnazyan Federal Medical Biophysical Center FMBA of Russia, Moscow, Russia

I. I Eremin

A. I. Burnazyan Federal Medical Biophysical Center FMBA of Russia, Moscow, Russia

V. A Rybko

N. N. Blokhin Cancer Research Center, Moscow, Russia

A. I Zorina

Human Stem Cells Institute, Moscow, Russia

K. V Kotenko

A. I. Burnazyan Federal Medical Biophysical Center FMBA of Russia, Moscow, Russia

A. A Pulin

A. I. Burnazyan Federal Medical Biophysical Center FMBA of Russia, Moscow, Russia

P. B Kopnin

Human Stem Cells Institute, Moscow, Russia; N. N. Blokhin Cancer Research Center, Moscow, Russia


  1. Meregalli М., Farini А., Sitziaand С. et al. Advancements in stem cells treatment of skeletal muscle wasting. Front Physiol. 2014; 5 (48): 1-12.
  2. McCullagh К., Perlingeiro R. Coaxing stem cells for skeletal muscle repair. Adv. Drug Deliv. Rev. 2014; pii: S0169-409X (14) 00148-3 [Epub ahead of print].
  3. Shi Х., Garry D. J. Muscle stem cells in development, regeneration, and disease. Genes Dev. 2006; 20: 1692-708.
  4. Cerletti M., Jurga S., Witczak C. A. et al. Highly efficient, functional engraftment of skeletal muscle stem cells in dystrophic muscles. Cell 2008; 134: 37-47.
  5. Carlson M.E., O'Connor M.S. Hsu M. et al. Notch signaling pathway and tissue engineering. Front Biosci. 2007;12: 5143-56.
  6. Farini A., Razini P., Erratico S. et al. Cell based therapy for Duchenne muscular dystrophy. J. Cell Physiol. 2009; 221 (3): 526-34.
  7. Dezawa M., Ishikawa H., Hoshino M. et al. Potential of bone marrow stromal cells in applications for neuro-degenerative, neuro-traumatic and muscle degenerative diseases. Curr. Neuropharmacol. 2005; 3 (4): 257-66.
  8. Фриденштейн А. Я., Чайлахян Р. К., Герасимов Ю. В. Пролиферативный и дифференцировочный потенциал скелетных костномозговых колониеформирующих клеток. Цитология 1986; 28 (3): 341-9.
  9. Caplan A. I. Mesenchymal stem cells. J. Orthop. Res. 1991; 9: 641-50.
  10. Bianco P., Robey P. G., Simmons P. J. Mesenchymal stem cells: revisiting history, concepts, and assays. Cell Stem Cell 2008; 2: 313-19.
  11. Hematti P. Mesenchymal stromal cells and fibroblasts: a case of mistaken identity? Cytotherapy 2012; 14: 516-21.
  12. Sorrell J. M., CaplanA. I. Fibroblast heterogeneity: more than skin deep. J. Cell Sci. 2004; 117: 667-75.
  13. Haniffa M. A., CollinM. P., Buckley C. D. et al. Mesenchymal stem cells: the fibroblasts' new clothes? Haematologica 2009; 94 (2): 258-63.
  14. Zuk P. A., Zhu M., Mizuno H. et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001;7 (2): 211-28.
  15. Oswald J., Boxberger S., Jorgensen B. et al. Mesenchymal stem cells can be differentiated into endothelial cells in vitro. Stem Cells 2004;22 (3): 377-84.
  16. Robey P.G. Cell sources for bone regeneration: the good, the bad, and the ugly (but promising). Tissue Engin. 2011; 17 (6): 423-30.
  17. Sekiya I., Larson B. L., Smith J. R. et al. Expansion of human adult stem cells from bone marrow stroma: conditions that maximize the yields of early progenitors and evaluate their quality. Stem Cells 2002; 20 (6): 530-41.
  18. Zorin V. L., Komlev V. S., Zorina A. I. et al. Octacalcium phosphate ceramics combined with gingiva-derived stromal cells for engineered functional bone grafts. Biomed. Mater. 2014; 9 (5): 055005.
  19. Sakaguchi Y., Sekiya I., Yagishita K. et al. Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source. Arthritis Rheum. 2005; 52 (8): 2521-9.
  20. Mitrano T. I., Grob M. S., Carrion F. et al. Culture and characterization of mesenchymal stem cells from human gingival tissue. J. Periodontol. 2010; 81 (6): 917-25.
  21. Zhang Q. Z., Nguyen A. L., Yu W. H. et al. Human oral mucosa and gingiva: a unique reservoir for mesenchymal stem cells. J. Dent. Res. 2012; 91 (11): 1011-8.
  22. Fournier B. P. J., Larjava H., Hakkinen L. Gingiva as a source of stem cells with therapeutic potential. Stem Cells Dev. 2013; 22 (24): 3157-77.
  23. Xu X., Chen C., Akiyama K. et. al. Gingivae contain neural-crest- and mesoderm-derived mesenchymal stem cells. J. Dent. Res. 2013;92 (9): 825-32.
  24. Marynka-Kalmani K., Treves S., Yafee M. et al. The lamina propria of adult human oral mucosa harbors a novel stem cell population. Stem Cells 2010; 28 (5): 984-95.
  25. Treves-Manusevitz S., Hoz L., Rachima H. et al. Stem cells of the lamina propria of human oral mucosa and gingiva develop into mineralized tissues in vivo. J. Clin. Periodontol. 2013; (40): 73-81.
  26. Tomar G. B., Srivastava R. K., Gupta N. et al. Human gingiva-derived mesenchymal stem cells are superior to bone marrow-derived mesenchymal stem cells for cell therapy in regenerative medicine. Biochem. Biophys. Res. Commun. 2010; 393 (3): 377-83.
  27. Huang G. T, Gronthos S., Shi S. Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J. Dent. Res. 2009; 88 (9): 792-806.
  28. Price F., Kuroda D., RudnickiM. A. Stem cell based therapies to treat muscular dystrophy. Biochim. Biophys. Acta. 2007; 1772 (2): 272-83.
  29. Mauro А. Satellite cell of skeletal muscle fibers. J. Biophys. Biochem. Cytol. 1961; 9: 493-5.
  30. Gussoni B., KunkelL. M. The fate of individual myoblasts after transplantation in to muscles of DMD patients. Nat. Med. 1997; 3: 970-7.
  31. Meligy F. Y., Shigemura K., Behnsawy H. M. et al. The efficiency of in vitro isolation and myogenic differentiation of MSCs derived from adipose connective tissue, bone marrow, and skeletal muscle tissue. In Vitro Cell Dev. Biol. Anim. 2012;48 (4): 203-15.
  32. Ferrari G., Cusella-De Angelis G., Coletta M. et al. Muscle regeneration by bone marrow-derived myogenic progenitors. Science 1998; 279 (5356): 1528-30.
  33. Зорин В. Л., Зорина А. И., Еремин И. И. и др. Сравнительный анализ остеогенного потенциала мультипотентныхмезенхи-мальныхстромальных клеток слизистой оболочки полости рта и костного мозга. Гены и клетки 2014;9 (1): 50-7.
  34. Dominici M., LeBlanc K., Mueller I. etal. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 2006; 8 (4): 315-7.
  35. Dezawa M., Ishikawa H., Itokazu Y. et. al. Bone marrow stromal cells generate muscle cells and repair muscle degeneration. Science 2005;309 (5732):314-7.

Copyright (c) 2014 Eco-Vector

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС 77 - 85657 от 21.07.2023 от 11.03.2014.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies