Application of genetically modified umbilical cord blood cells and direct gene therapy for treatment of skin wounds in rats


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Abstract

There were proposed several methods for stimulation of skin wound repair over the last few decades. The most perspective among them are gene and stem cell therapy. In our experiments we combined both approaches by application of human cord blood mononuclear cells (hUCB-MC) transfected with pBud-VEGF165-FGF2 plasmid to enhance healing of full thickness skin wounds in rats. Dual expression cassette plasmid pBud-VEGF165-FGF2 encodes both VEGF and FGF2 therapeutic genes, expressing pro-angiogenic growth factors. Our results showed that in 2 weeks after transplantation some transplanted cells still retain expression of stem cell and hematopoietic markers C-kit and CD34. Other transplanted cells could be found among keratinocytes, hair follicle cells, endothelial cells and in derma. Study of PCNA expression revealed that application of transfected cells terminate proliferative processes in regenerating wound earlier then application of untransfected cells.

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About the authors

I. M Gazizov

Kazan (Volga region) Federal University; Kazan State Medical University

I. I Salafutdinov

Kazan (Volga region) Federal University

M. O Mavlikeev

Kazan (Volga region) Federal University

F. V Bashirov

Kazan State Medical University

R. R Islamov

Kazan State Medical University

A. A Rizvanov

Kazan (Volga region) Federal University

A. P Kiassov

Kazan (Volga region) Federal University

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