Invasive ability of proteus mirabilis and morganella morganii



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Abstract

Here we discuss the ability of Proteus mirabilis 5127-1 and Morganella morganii ZM to invade human cervical epithelial adenocarcinoma cells (HeLa-M) and to cause cytotoxicity in those cells. We found that ability of bacteria to invade HeLa-M cells depended on the multiplicity of infection for both strains. Cytotoxicity of P. mirabilis on HeLa-M cells was evaluated through quantification of eukaryotic cell detachment from plastic. We hypothesized that cytotoxicity of P.mirabilis could be due its extracellular proteolytic and hemolytic activities. In contrast, no cytotoxicity was detected in HeLa-M cells infected with M.morganii.

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About the authors

N. M Zamaliutdinova

Kazan (Volga region) Federal University

L. B Bogomolnaya

Kazan (Volga region) Federal University

I. B Chastukhina

Kazan (Volga region) Federal University

M. R Sharipova

Kazan (Volga region) Federal University

A. M Mardanova

Kazan (Volga region) Federal University

References

  1. Okamura N., Nagai T., Nakaya R. et al. HeLa cell invasiveness and О antigen of Shigella flexneri as separate and prerequisite attributes of virulence to evoke keratoconjunctivitis in guinea pigs. Infect. Immun. 1983; 39: 505-13.
  2. Mehlham I.J., Eide E.L., Sanders A.C. et al. Methodology for recognition of invasive potential of Escherichia coli. J. Assoc. Anal. Chem. 1977; 60: 546-62.
  3. Kihlstrom E. Infections of HeLa cells with Salmonella typhimurium 395 MS and MR10 bacteria. Infect. Immun. 1977; 17: 290-5.
  4. Peerbooms P.G.H., Verweij A.M.J.J., MacLaren D.M. Vero Cell Invasiveness of Proteus mirabilis. Infect. Immun. 1984; 43(3): 1068-71.
  5. Gorrill R.H., DeNavasquez S.J. Experimental pyelonephritis in the mouse produced by Escherichia coli, Pseudomonas aeruginosa and Proteus mirabilis. J. Pathol. Bacteriol. 1964; 87: 79-87.
  6. Endimiani A., Luzzaro F., Brigante G. et al. Proteus mirabilis bloodstream infections: risk factors and treatment outcome related to the expression of extended-spectrum beta-lactamases. Antimicrob. Agents Chemother. 2005; 49(7): 2598-605.
  7. Jakobsen S.M., Stickler D.J., Mobley H.L.T. et al. Complicated catheter-associated urinary tract infections due to Escherichia coli and Proteus mirabilis. Clin. Microbiol. Rev. 2008; 21(1): 26-59.
  8. Torzewska A., Budzynska A., Bialczak-Kokot M. et al. In vitro studies of epithelium-associated crystallization caused by uropathogens during urinary calculi development. Microb. Pathog. 2014; 71-2: 25-31.
  9. Mathoera R.B., Kok D.J., Verduin C.M. et al. Pathological and therapeutic significance of cellular invasion by Proteus mirabilis in an enterocystoplasty infection stone model. Infect. Immun. 2002; 70(12): 7022-32.
  10. Peerbooms P.G.H., Verweij A.M.J.J., MacLaren D.M. Vero Cell Invasiveness of Proteus mirabilis. Infect. Immun. 1984; 43(3): 1068-71.
  11. Allison C., Coleman N., Jones P.L. et al. Ability of Proteus mirabilis to invade human urothelial cells is coupled to motility and swarming differentiation. Infect. Immun. 1992; 60(11): 4740-6.
  12. Kihlstrom E. Infections of HeLa cells with Salmonella typhimurium 395 MS and MR10 bacteria. Infect. Immun. 1977; 17: 290-5.
  13. Larsson P., Oiling S. О-antigen distribution and sensitivity to the bactericidal effect of normal human serum of Proteus strains from clinical specimens. Med. Microbiol. Immunol. 1977; 163: 77-82.
  14. Schiemann D.A., Devenish J.A. Relationship of HeLa cell infectivity to biochemical, serological and virulence characteristics of Yersinia enterocolitica. Infect. Immun. 1982; 35: 497-506.
  15. Sambrook J., Fritsch E.F., Maniatis T. Molecular cloning: a laboratory manual. 2nd ed. NY: Cold Spring Harbor Laboratory Press; 1989.
  16. Bozhokina E.S., Tsaplina O.A., Efremova T.N. et al. Bacterial invasion of eukaryotic cells can be mediated by actin-hydrolysing metalloproteases grimelysin and protealysin. Cell Biol. Int. 2011; 35: 111-8.
  17. Шамов О.В., Орлов Д.С., Пазина Т.Ю. и др. Изучение молекулярно-клеточных основ цитотоксического действия антимикробных пептидов на опухолевые клетки. Фундаментальные науки. Биологические исследования 2012; 5: 207-12.
  18. Замалютдинова Н.М., Галиева И.Р., Арапова А.О. и др. Протеолитическая активность бактерий трибы Proteae. Вестник КГТУ 2013; 10: 191-4.
  19. Pearson M.M., Sebaihia M., Churcher C. et al. Complete genome sequence of uropathogenic Proteus mirabilis, a master of both adherence and motility. Bacteriology 2008; 190: 4027-37.
  20. Wang R., Neoh K.G., Kang E.T. et al. Antifouling coating with controllable and sustained silver release for long-term inhibition of infection and encrustation in urinary catheters. J. Biomed. Mater. Res. B. Appl. Biomat. 2014; doi: 10.1002/jbm.b.33230.
  21. Kochiashvili D., Khuskivadze A., Kochiashvili G. et al. Role of the bacterial vaccine solco-urovac in treatment and prevention of recurrent urinary tract infections of bacterial origin. Georg. Med. News. 2014; 231: 11-6.
  22. Alamuri P., Mobley H.L.T. A novel autotransporter of uropathogenic Proteus mirabilis is both a cytotoxin and an agglutinin. Mol. Microbiol. 2008; 68(4): 997-1017.
  23. Mittal R., Grati1 M., Gerring R. et al. In vitro interaction of Pseudomonas aeruginosa with human middle ear epithelial cells. Plos One 2014; 9(3): e91885.
  24. Lima C.B.C., Santos S.A., Andrade D.R.Jr. Hypoxic stress, hepatocytes and CACO-2 viability and susceptibility to Shigella Flexneri invasion. Rev. Inst. Med. Trop. Sao Paulo. 2013; 55(5): 341-6.
  25. Kothary M.H., McCardell B.A., Frazar C.D. et al. Characterization of the zinc-containing metalloprotease encoded by zpx and development of a species-specific detection method for Enterobacter sakazakii. Appl. Environ. Microbiol. 2007; 73(13): 4142-51.
  26. Lockwood D.E., Kreger A.S., Richardson S.H. Detection of toxins produced by Vibrio fluvialis. Infect. Immun. 1982; 35(2): 702-8.

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