Decellularized human umbilical arteries retain their mechanical properties

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Abstract

Tissue engineered vascular grafts can fulfill a clinical need in biological prostheses in reconstructive cardiovascular surgery. Decellularized arteries do not cause immune response, are biocompatible, could be reseeded with recipient cells und thus are attractive scaffolds for vascular tissue engineering. Earlier we developed a decullarization method for human umbilical arteries and proved its effectiveness morphologically. The purpose of this study was to evaluate mechanical properties of the decellurized human umbilical arteries, also after long-term storage. 3 groups of vessels were investigated: I group - native arteries, II group - decellularized arteries, III group - decellularized arteries, stored in phosphate buffered saline for 10 months. Samples were stretched until rupture on the Instron universal testing machine; herewith strain and stress were recorded. The same way the suture retention strength was estimated. Also burst-pressure, that characterized the total strength of the samples, was investigated. Intergroup differences in maximum strain both longitudinal and transverse directions, suture retention strength and burst pressure were not significant. Decellularized human umbilical arteries retain their mechanical properties, and that indirectly confirms extracellular matrix preservation. Thus they are attractive source for small diameter tissue engineered vascular grafts.

About the authors

A. S Nasredinov

Federal Almazov Medical Research Centre, Saint-Petersburg, Russia

A. V Lavreshin

Federal Almazov Medical Research Centre, Saint-Petersburg, Russia

E. A Lebedeva

Saint-Petersburg Electrotechnical University «LETI», Saint-Petersburg, Russia

S. V Anisimov

Federal Almazov Medical Research Centre, Saint-Petersburg, Russia

V. N Vavilov

Federal Almazov Medical Research Centre, Saint-Petersburg, Russia

D. I Kurapeev

Federal Almazov Medical Research Centre, Saint-Petersburg, Russia

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