Autologous dermal fibroblasts stimulate regeneration of degenerative Achilles tendon

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access


Cell therapy with applying autologous skin fibroblasts could be an alternative or additional way to the standard therapy of tendon degenerative changes. The research was aimed to study a therapeutic effect of local administration of cultured and cryopreserved autologous skin fibroblasts in dynamics under tendopathy. Rats with modeled а^!^ tendon degenerative and dystrophic injury received by 0.25х10 6 of native or cryopreserved autologous skin fibroblasts into the defect area. The animals with administered physiological saline served as the control. 21 and 45 days after therapy there were implemented the histological, immunofluorescent and biomechanical studies. The data analysis testified to the fact, that cultured and cryopreserved autologous skin fibroblasts contributed to activating reparative processes in damaged tendons. On the first time point (21 day) the application of cryopreserved cells resulted in less pronounced therapeutic effect as compared to cultured fibroblasts. To the 45 day of study in аchilles tendons' histological structure of animals received both cultured and cryopreserved autologous skin fibroblasts we observed the presence of cell elements, reduction of disorganization phenomena, normalization of structure and position of collagen I type fibers. In addition, the animals' tendons with therapy by cultured and cryopreserved autologous skin fibroblasts had a positive dynamics in biomechanical indices: we observed a gradual positive strength recovery during tension, which to the 45 day of therapy did not statistically and significantly differ from the corresponding indices in the intact animals.

About the authors

N. A Volkova

Institute of Traumatology and Orthopaedics of NAMS of Ukraine, Kiev, Ukraine

M. S Yukhta

Institute of Traumatology and Orthopaedics of NAMS of Ukraine, Kiev, Ukraine

R. I Blonskiy

nstitute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine, Kharkov, Ukraine

A. A Kostrub

nstitute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine, Kharkov, Ukraine

A. N Goltsev

Institute of Traumatology and Orthopaedics of NAMS of Ukraine, Kiev, Ukraine


  1. Weinfeld S.B. Achilles tendon disorders. Med. Clin. North Am. 2014; 98: 331-8.
  2. Graham R. Tendinopathy - from basic science to treatment. Nature Clinical Practice Rheum. 2008; 4: 82-9.
  3. Jacobsson J. Towards systematic prevention of athletics injuries: use of clinical epidemiology for evidence based injury prevention. Sweden [Linkoping]: LiU-Tryck; 2012.
  4. Richardson L.E., Dudhia J., Clegg P.D. et. al. Stem cells in veterinary medicine - attempts at regenerating equine tendon after injury. Trends Biotech. 2007; 25: 409-16.
  5. Lui P.P. Cell therapy for the treatment of tendinopathy - a systematic review on the pre-clinical and clinical evidence. Semin. Arthritis Rheum. 2013; 42: 651-66.
  6. Зорина А.И., Бозо И.Я., Зорин В.Л. и др. Фибробласты дермы: особенности цитогенеза, цитофизиологии и возможности клинического применения. КТТИ 2011; 6(2): 16-26.
  7. Масгутов Р.Ф., Салихов Р.З., Плаксейчук Ю.А. и др. Применение клеток стромальной васкулярной фракции жировой ткани при ложном суставе бедренной кости: клинический случай. КТТИ 2013; 8(3): 116-8.
  8. Smith R.K., Werling N.G., Dakin S.G. et. al. Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy. PLoS One 2013; 8(9): e75697.
  9. Бозо И.Я., Деев Р.В., Пинаев Г.П. «Фибробласт» - специализированная клетка или функциональное состояние клеток мезенхимного происхождения? Цитология 2010; 52(2): 99-109.
  10. Гончаров В.П. Факторы роста фибробластов. Физиол. журнал 1994; 9: 163-73.
  11. Alfaro M.P., Deskins D.L., Wallus M. et al. A physiological role for connective tissue growth factor in early wound healing. Lab. Invest. 2013; 93(1): 81-95.
  12. Guo Y., Zeng Q., Yan Y. et al. Proliferative effect and osteoinductive potential of extracellular matrix coated on cell culture plates. Springerplus 2013; 2: 303.
  13. Зорин В.Л., Зорина А.И., Петракова О.С. и др. Дермаль-ные фибробласты для лечения дефектов кожи. КТТИ 2009; 4(4): 26-40.
  14. Haack-Sorensen M., Bindslev L., Mortensen S. et al. The influence of freezing and storage on the characteristics and functions of human mesenchymal stromal cells isolated for clinical use. Cytotherapy 2007; 9(4): 328-37.
  15. Council of Europe [France]. Europiane convention for the proyection of vertebrate animals used for experimental and other scientific purposes. Strasburg, 18. III. 1986; http:// convention. coe. Int/ treaty/ en/Treaties/Word/ 123.doc.
  16. Адамс Р. Методы культуры клеток для биохимиков. М.: Мир; 1983.
  17. Csizy M., Hintermann B. Rupture of the Achilles tendon after local steroid injection. Case reports and consequences for treatment. Swiss Surg. 2001; 7(4): 184-9.
  18. Tatari H., Skiak E., Destan H. et al. Effect of hylan G-F 20 in Achilles' tendonitis: an experimental study in rats. Arch. Phys. Med. Rehabil. 2004; 85(9): 1470-4.
  19. Lin T.E., Lakhiani C., Lee M.R. Biomechanical analysis of knotless flexor tendon repair using large-diameter unidirection barbed suture. Hand (N.Y.) 2013; 8(3): 315-9.
  20. Egemen O., Ozkaya O., Ozturk M.B. et al. The biomechanical and histological effects of diabetes on tendon healing: experimental study in rats. J. Hand Microsurg. 2012; 4(2): 60-4.
  21. Nolte S.V., Xu W., Rennekampff H.O., Rodemann H.P. Diversity of fibroblasts - a review on implications for skin tissue engineering. Cells Tissues Organs 2008; 187 (3): 165-76.
  22. Francavilla C., Rigbolt K.T., Emdal K.B. et al. Functional proteomics defines the molecular switch underlying FGF receptor trafficking and cellular outputs. Molecular Cell 2013; 51(6):707-22.
  23. Jiang Ch., Shao L., Wang Q., Dong Y. Repetitive mechanical stretching modulates transforming growth factor-p induced collagen synthesis and apoptosis in human patellar tendon fibroblasts. Biochemistry and Cell Biology 2012; 90(5): 667-74.
  24. Lee S.H., Jin S.Y., Song J.S. et al. Paracrine effects of adipose-derived stem cells on keratinocytes and dermal fibroblasts. Ann. Dermatol. 2012; 24(2): 136-43.
  25. Jo C.H., Kim J.E., Yoon K.S. et al. Does platelet-rich plasma accelerate recovery after rotator cuff repair? A prospective cohort study. Am. J. Sports Med. 2011; 39(10): 2082-90.
  26. Muttini A., Salini V., Valbonetti L., Abate M. Stem cell therapy of tendinopathies: suggestions from veterinary medicine. Muscl., Ligam. Tend. J 2012; 2(3): 187-192.
  27. Obaid H., Clarke A., Rosenfeld P. et al. Skin-derived fibroblasts for the treatment of refractory Achilles tendinosis: preliminary shortterm results. J. Bone Joint Surg. Am. 2012; 94(3): 193-200.

Copyright (c) 2014 Eco-Vector

СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: ПИ № ФС 77 - 85657 от 21.07.2023 от 11.03.2014.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies