Autologous dermal fibroblasts stimulate regeneration of degenerative Achilles tendon

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Abstract

Cell therapy with applying autologous skin fibroblasts could be an alternative or additional way to the standard therapy of tendon degenerative changes. The research was aimed to study a therapeutic effect of local administration of cultured and cryopreserved autologous skin fibroblasts in dynamics under tendopathy. Rats with modeled а^!^ tendon degenerative and dystrophic injury received by 0.25х10 6 of native or cryopreserved autologous skin fibroblasts into the defect area. The animals with administered physiological saline served as the control. 21 and 45 days after therapy there were implemented the histological, immunofluorescent and biomechanical studies. The data analysis testified to the fact, that cultured and cryopreserved autologous skin fibroblasts contributed to activating reparative processes in damaged tendons. On the first time point (21 day) the application of cryopreserved cells resulted in less pronounced therapeutic effect as compared to cultured fibroblasts. To the 45 day of study in аchilles tendons' histological structure of animals received both cultured and cryopreserved autologous skin fibroblasts we observed the presence of cell elements, reduction of disorganization phenomena, normalization of structure and position of collagen I type fibers. In addition, the animals' tendons with therapy by cultured and cryopreserved autologous skin fibroblasts had a positive dynamics in biomechanical indices: we observed a gradual positive strength recovery during tension, which to the 45 day of therapy did not statistically and significantly differ from the corresponding indices in the intact animals.

About the authors

N. A Volkova

Institute of Traumatology and Orthopaedics of NAMS of Ukraine, Kiev, Ukraine

M. S Yukhta

Institute of Traumatology and Orthopaedics of NAMS of Ukraine, Kiev, Ukraine

R. I Blonskiy

nstitute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine, Kharkov, Ukraine

A. A Kostrub

nstitute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine, Kharkov, Ukraine

A. N Goltsev

Institute of Traumatology and Orthopaedics of NAMS of Ukraine, Kiev, Ukraine

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